Recent Development of Aptasensor for Influenza Virus Detection

A SPR Aptasensor for Detection of Avian Influenza Virus H5N1

Rapid and specific detection of avian influenza virus (AIV) is urgently needed due to the concerns over the potential outbreaks of highly pathogenic H5N1 influenza in animals and humans. Aptamers are artificial oligonucleic acids that can bind specific target molecules, and show comparable affinity for target viruses and better thermal stability than monoclonal antibodies. The objective of this...

Standardization of ELISA for Detection of Avian Influenza Virus Antibodies

Immunofluorescence Method for The Detection of Non-Pathogenic Influenza Virus

The direct F.A. Method has been used for diffe­rentiation of virulent and avirulent lines of influenza viruses. A positive fluorescence was obtained indi­cating the level of virus in the tissues, sorbing with dried mouse powder helped to reduce non-specific staining. The virulent line showed the greatest num­ber of fluorescent cells with the peak at 10 hours. the non-virulent lines showed a gra...

QCM Aptasensor for Rapid and Specific Detection of Avian Influenza Virus

There has been a need for rapid detection of Avian Influenza virus (AIV) H5N1 due to it being a potential pandemic threat. Most of the current methods, including culture isolation and PCR, are very sensitive and specific but require specialized laboratories and trained personnel in order to complete the tests and are time-consuming. The goal of this study was to design a biosensor that would be...

An Impedance Aptasensor with Microfluidic Chips for Specific Detection of H5N1 Avian Influenza Virus

In this research a DNA aptamer, which was selected through SELEX (systematic evolution of ligands by exponential enrichment) to be specific against the H5N1 subtype of the avian influenza virus (AIV), was used as an alternative reagent to monoclonal antibodies in an impedance biosensor utilizing a microfluidics flow cell and an interdigitated microelectrode for the specific detection of H5N1 AI...